赵小凡 教授
博士生导师
电话；0531-88364620
E-mail:xfzhao#sdu.edu.cn
教育背景
从事昆虫生理生化与分子生物学研究，重点研究昆虫发育过程中蜕皮与变态的功能基因和分子调控机理。承担本科生和研究生教学工作。1990-1991年赴日本从事家蚕半胱氨酸蛋白酶研究；1998-2000年获德国政府学术交流会（DAAD）生物科学特别项目博士后奖学金赴德国从事烟草天蛾V-ATP酶研究；2001-2002年在日本九州工业大学从事DNA和蛋白质测序、基因重组蛋白包涵体复性和纯化、毕赤酵母表达外源基因等研究；2003年获德国政府德意志联合研究会（DFG）资助赴德国从事基因重组病毒杀虫剂构建和蛋白酶、HHR3在昆虫细胞中的表达等研究。2005年赴日本从事蛋白质组学研究。2006年赴美国密苏里大学进行短期科研工作。先后承担国家自然科学基金重点项目、重大国际合作项目和面上项目；参加国家973和863项目；以及承担山东省自然科学基金重点项目等科研项目10余项。主要学术成果有：从棉铃虫鉴定并纯化到组织蛋白酶B，克隆到该蛋白酶基因（HCB），阐明了该蛋白酶在棉铃虫生长发育中的功能，从大豆等植物种子中鉴定并纯化到该蛋白酶的抑制因子，对肿瘤细胞有抑制生长的作用；克隆了棉铃虫蜕皮调节转录因子HHR3基因并阐明了该基因的表达和激素调控模式；阐明了多种棉铃虫幼虫蜕皮和变态时期差异表达的蛋白质和基因，为研究昆虫发育的分子机制提供了新靶标；证明了非甾醇类蜕皮激素竞争物（RH-2485）可以导致棉铃虫致死性提前蜕皮，可用于田间控制棉铃虫；构建了HCB和HHR3基因重组核型多角体病毒，可用于开发高技术生物杀虫剂用于害虫控制。获得国家发明专利多项。济南市第十二届、第十三届、第十四届人大代表。
研究方向
研究生招生专业：生化与分子生物学
研究方向：昆虫生化与分子生物学
研究内容：以棉铃虫为材料，用分子生物学方法研究昆虫蜕皮与变态的分子机理，包括：蜕皮激素信号转导途径、蜕皮与变态功能基因的表达与调控、变态发育中组织重建的分子机理、血细胞在变态发育中的功能等。
Research interest: We use the cotton bollworm (Helicoverpa armigera) as a model to investigate the mechanism of insect molting and metamorphosis by molecular methods. Recent studies include: 20-OH-ecdysteroid hormone signal transduction pathway; expression and regulation of molting and metamorphosis related genes; molecular mechanism of tissue remodeling; and function of haemocytes in molting and metamorphosis.
在研课题
1. 昆虫血细胞在变态中的功能和作用机理,国家自然科学基金。
2. 害虫暴发成灾的遗传与行为机理，国家重大基础研究项目973。
已完成课题
14. 棉铃虫皮层溶离中蛋白酶表达及调控研究，国家自然科学基金,2007.1-2009.12。项目负责人。
13. 重大农业害虫猖獗危害的机制及可持续控制的基础研究——害虫生长发育与生殖调控的分子机制。国家重大基础研究项目（973）。2006.1-2010.12。参加。
12. 家蚕重要经济性状功能基因克隆，国家高技术研究发展计划（863计划）2006. 1-2010.12。参加。
11．昆虫蜕皮激素信号转导途径研究,国家自然科学基金重大国际合作项目,2008.1-2011.12月。项目负责人。
10. 棉铃虫蜕皮级联反应功能基因表达研究,国家自然科学基金重点项目，2004.1-2007.12。项目负责人。
9. 基因重组病毒杀虫剂研究,山东省自然科学基金重点项目,2004.1-2006.12. 项目负责人。
8. 重组病毒杀虫剂的研制，863，国家科技部,2001-2004. 项目负责人。
7. 棉铃虫蜕皮调节转录因子基因克隆和表达载体构建,国家自然科学基金,2001.1-2003.1. 项目负责人。
6. 棉铃虫卵蛋白酶基因克隆及功能表达,国家自然科学基金,1999.1-2001.12. 项目负责人。
5. RH-化合物对棉铃虫的防治效果研究,山东省科委国际合作基金,1998.1-2000.12. 项目负责人。
4. 蜕皮激素类似物合成及对棉铃虫的防治研究,山东省科委优秀年轻教师奖励基金,1999.1-2000.12. 项目负责人。
3. 棉铃虫卵内蛋白酶纯化,国家自然科学基金主任基金,1998.1-12. 项目负责人。
2. 鳞翅目昆虫卵内蛋白酶纯化性质与cDNA克隆. 国家教育部优秀年轻教师基金,1996.1-1998.12. 项目负责人。
1. 蓖麻蚕卵黄蛋白质降解酶研究，国家教育部留学回国人员基金，1992.1-1994.12. 项目负责人。
研究成果及发表论文
代表性论文：*为通讯作者，下划线为并列作者。
63. Wang Chuan-Xu,Zheng Wei-Wei,Liu Peng-Cheng,Wang Jin-Xing,Zhao Xiao-Fan*,2012,The steroid hormone 20-hydroxyecdysone upregulated the protein phosphatase 6 for the programmed cell death in the insect midgut. Amino Acids. DOI 10.1007/s00726-011-1159-9.
62. Dong Du-Juan,Liu Peng-Cheng,Wang Jin-Xing,Xiao-Fan Zhao*,2012,The knockdown of Ha-GRIM-19 by RNA interference induced programmed cell death. Amino Acids. 42(4):1297-307.
61. He Hong-Juan,Hou Li,Wang Jin-Xing,Zhao Xiao-Fan*,2012,The apoptosis inhibitor survivin prevents insect midgut from cell death during postembryonic development. Molecular Biology Report. 39(2):1691-9.
60. Zhang Feng-Xia,Shao Hong-Lian,Wang Jin-Xing,Zhao Xiao-Fan*,2011,b-thymosin is upregulated by the steroid hormone 20-hydroxyecdysone and microorganisms.Insect Molecular Biology. 20,519–527.
59. Liu Peng-Cheng,Wang Jin-Xing,Song Qi-Sheng,Zhao Xiao-Fan*,2011,The participation of calponin in the crosstalk between 20-Hydroxyecdysone and juvenile hormone signaling pathways by phosphorylation variation. PLoS ONE. 6: e19776.
58. Wang Gang,Liu Peng-Cheng,Wang Jin-Xing,Zhao Xiao-Fan*,2011,A BTBdomain-containinggeneis upregulatedby immunechallenge. Archives of Insect Biochemistry and Physiology. 77:58-71.
57. Yang Xiao-Hui,Liu Peng-Cheng,Zheng Wei-Wei,Wang Jin-Xing,Zhao Xiao-Fan*,2011,The juvenile hormone analogue methoprene up-regulates the Ha-RNA-binding protein. Molecular and Cellular Endocrinology 333: 172–180.
56. Hou Li,Wang Jin-Xing,Zhao Xiao-Fan*,2010,Rab32 and the remodeling of the imaginal midgut in Helicoverpa armigera. Amino Acids,DOI 10.1007/s00726-010-0720-2.
55. Wang Qian,Liu Yang,He Hong Juan,Zhao Xiao-Fan*,Jin-Xing Wang*,2010,Immune response of Helicoverpa armigera to different kinds of pathogens. BMC Immunology. 11:9.
54. Wang Jia-Lin,Wang Li-fu,Wang Jin-Xing,Zhao Xiao-Fan*,2010,The expression patterns of a eukaryotic initiation factor 3 subunit h in the silk glands in Bombyx mori. Archives of insect biochemistry and physiology. 75:1-12.
53. He Hong-Juan,Wang Qian,Zheng Wei-Wei,Wang Jin-Xing,Song Qi-Sheng,Zhao Xiao-Fan*,2010,Function of Nuclear Transport Factor 2 and Ran in the 20E signal transduction pathway in the Cotton Bollworm,Helicoverpa armigera. BMC Cell Biology. 11:1.
52. Wang Li-Fu,Chai Lian-Qin,He Hong-Juan,Wang Qian,Wang Jin-Xing,Zhao Xiao-Fan*,2010,The cathepsin L-like proteinase is involved in molting and metamorphosis in Helicoverpa armigera. Insect Molecular Biology,19:99-111.
51. Zheng Wei-Wei,Yang Dan-Tong,Wang Jin-Xing,Song Qi-Sheng,Gilbert Lawrence I.,Zhao Xiao-Fan*,2010,Hsc70 binds to ultraspiracle resulting in the upregulation of 20-hydroxyecdsone-responsive genes in Helicoverpa armigera. Molecular and Cellular Endocrinology. 315: 282-291.
50. Fu Qiang,Liu Peng-Cheng,Wang Jin-Xing,Song Qi-Sheng,Zhao Xiao-Fan*,2009,Proteomic identification of differentially expressed and phosphorylated proteins in epidermis involved in larval-pupal metamorphosis of Helicoverpa armigera. BMC Genomics. 10:600.
49. Liu Yang,Sui Yi-Peng,Wang Jin-Xing and Zhao Xiao-Fan*,2009,Characterization of the trypsin-like protease (ha-tlp2) constitutively expressed in the integument of the cotton bollworm,Helicoverpa armigera. Archives of Insect Biochemistry and Physiology. 72:74-87.
48. Sui Yi-Peng,Wang Jin-Xing and Zhao Xiao-Fan*,2009,The impacts of classical insect hormones on the expression profiles of a new digestive trypsin-like protease (TLP) from the cotton bollworm,Helicoverpa armigera. Insect Molecular Biology. 18: 443-452.
47. Sui Yi-Peng,Liu Xiao-Bin,Chai Lian-Qin,Wang Jin-Xing,Zhao Xiao-Fan*,2009,Characterization and influences of classical insect hormones on the expression profiles of a molting carboxypeptidase A from the cotton bollworm (Helicoverpa armigera). Insect Molecular Biology,18: 353–363.
46. Dong Du-Juan,Wang Jin-Xing,Zhao Xiao-Fan*. 2009,A eukaryotic initiation factor 5C is upregulated during metamorphosis in the cotton bollworm,Helicoverpa armigera. BMC Developmental Biology. 9:19.
45. Wang Jia-Lin,Zhang Ya-Ping,Gu Yin-Yu,Wang Jin-Xing and Zhao Xiao-Fan*,2009,Function of a TGF-inducible nuclear protein in the silk gland in Bombyx mori. Insect Molecular Biology.18: 243–251.
44. Tian Yuan-Yuan,Liu Yang,Zhao Xiao-Fan*,Wang Jin-Xing*,2009,Characterization of a C-type lectin from the cotton bollworm,Helicoverpa armigera. Developmental and Comparative Immunology. 33:772–779.
43. Shao Hong-Lian,Zheng Wei-Wei,Liu Peng-Cheng,Wang Qian,Wang Jin-Xing,Zhao Xiao-Fan*,2008,Establishment of a New Cell Line from Lepidopteran Epidermis and Hormonal Regulation on the Genes. PLoS ONE 3: e3127.
42. Sui Yi-Peng,Wang Jin-Xing and Zhao Xiao-Fan*,2008,Effects of classical insect hormones on the expression profiles of a lipase gene from the cotton bollworm (Helicoverpa armigera). Insect Molecular Biology. 17: 523 – 529.
41. Shao Hong-Lian,Dong Du-Juan,Hu Jin-Dong,Wang Jin-Xin,Zhao Xiao-Fan*,2008,Construction of the recombinant baculovirus AcMNPV with cathepsin B-like proteinase and its insecticidal activity against Helicoverpa armigera. Pesticide Biochemistry and Physiology. 91:141-161.
40. Wang Jia-Lin,Wang Jin-Xing，Zhao Xiao-Fan*,2008,Molecular Cloning and expression Profiles of the Acyl-CoA-Binding Protein Gene From the Cotton Bollworm Helicoverpa armigera. Archives of Insect Biochemistry and Physiology. 68:79–88.
39．Chai Lian-Qin,Tian Yuan-Yuan,Yang Dan-Tong,Wang Jin-Xing,Zhao Xiao-Fan*,2008,Molecular cloning and characterization of a C-type lectin from the cotton bollworm,Helicoverpa armigera. Developmental Comparative Immunology,32:71-83.
38．Yang Dan-Tong,Chai Lian-Qin,Wang Jin-Xing,Zhao Xiao-Fan*,2008,Molecular cloning and characterization of Hearm caspase-1 from Helicoverpa armigera. Molecular Biology Report. 35:405-12.
37. Yang Xiao-Mei,Hou Li-Jing,Wang Jin-Xing,Zhao Xiao-Fan*,2007,expression and Function of Cathepsin B-Like Proteinase in Larval Hemocytes of Helicorverpa armigera during metamorphosis. Archives of Insect Biochemistry and Physiology. 64:164–174.
36. Hu Jindong,Shao Honglian,Zhang Yubao,Fu Qiang,Sun Chen，Wang Jinxing,Zhao Xiaofan*，2007，Construction of HaSNPV with Helicoverpa hormone receptor 3. High Technology Letters,3：327-331.
35. Shao Hong-Lian，Dong Du-Juan，Hu Jin-Dong，Zhang Xin-Chang，Zhang Yu-Bao，Fu Qiang，Sun Chen，Wang Jin-Xin，Zhao Xiao-Fan*,2007,Reconstruction of AcMNPV with Helicoverpa hormone receptor 3 and its effect on the Helicoverpa larvae. Biocontrol Science and Technology. 17: 95-104.
34. Dong Du-Juan1,He Hong-Juan1,Chai Lian-Qin1,Jiang Xiao-Juan,Wang Jin-Xing,Zhao Xiao-Fan*,2007,Identification of genes differentially expressed during larval molting and metamorphosis of Helicoverpa armigera. BMC Developmental Biology,7:73.
33．Wang Jia-Lin,Jiang Xiao-Juan,Wang Qian,Hou Li–Jing,Xu Da-Wei,Wang Jin-Xing,Zhao Xiao-Fan*,2007,Identification and expression profile of a putative basement membrane protein gene in the midgut of Helicoverpa armigera. BMC Developmental Biology,7:76.
32. Zhang Xin-Chang,Shao Hong-Lian,Wang Jin-Xing,Zhao Xiao-Fan*,2006,Purification of an inhibitor of Helicoverpa cathepsin B-like proteinase from sunflower seed. Journal of Enzyme Inhibition and Medicinal Chemistry,21: 433–439.
31. Yang Xiao-Mei,Hou Li-Jing,Dong Du-Juan,Shao Hong-Lian,Wang Jin-Xing,Zhao Xiao-Fan*,2006,Cathepsin B-Like Proteinase Is Involved in the Decomposition of the Adult Fat Body of Helicoverpa armigera. Archives of Insect Biochemistry and Physiology,62:1-10.
30. Zhao Xiao-Fan*,He Hong-Juan,Dong Du-Juan,Wang Jin-Xing. 2006,Identification of Differentially Expressed Proteins during Larval Molting of Helicoverpa armigera. Journal of Proteome Research,5:164-169.
29．Zhao Xiao-Fan*,An Xiao-Meng,Wang Jin-Xing,Dong Du-Juan,Du Xin-Jun,Sueda S. and Kondo H.,2005，expression of Helicoverpa cathepsin B-like proteinase during embryonic development，Archives of Insect Biochemistry and Physiology,58: 39-46.
28．Zhao Xiao-Fan*,Wang Jin-Xing,Li Fei-Xue,Sueda Shinji,Kondo Hiroki. 2005，Analysis of substrate specificity and endopeptidyl cleavage sites of the cathepsin B-like proteinase from Helicoverpa armigera,The Protein Journal,24:219 – 225.
27．Zhao Xiao-fan*,Wang Jin-Xing,Xu Xia-Lian,Li Zhao-Min,Kang Cui-Jie,2004，Molecular cloning and expression patterns of molt-regulating transcription factor-hhr3 from Helicoverpa armigera. Insect Molecular Biology.13:407-412.
26. Zhao Xiaofan*,Li Zhaomin,Wang Jinxing,Wang Jiabao,Wang Shengli,Blankespoor D. Harvey,2003,Efficacy of RH-2485,a new non-steroidal ecdysone agonist,against cotton bollworm,Helicoverpa armigera (lepidoptera: noctuidae) in laboratory and field. Crop Protection. 22：959-965.
25. Zhao X-F*,Wang J-X,Xu X-L,Schmid R.,Wieczorek H.,2002，Molecular cloning and characterization of the cathepsin B-like proteinase from the cotton boll worm,Helicoverpa armigera. Insect Molecular Biology. 11: 567-575.
24. Merzendorfer H.，Reineke S.，Zhao X-F,Jacobmeier B.,Harvey W.R.,Wieczorek H.,2000，The multigene family of the tobacco hornworm V-ATPase: novel subunits a,C,D,H,and putative isoforms. Biochemica et Biophysica Acta. 1467:369-379.
23．Zhao Xiao-Fan*,Wang Jin-Xing,Wang Yin-Chang，1998,Purification and characterization of a cysteine proteinase from eggs of the cotton boll worm,Helicoverpa armigera. Insect Biochemistry and Molecular Biology. 28:259-264.
22. Zhao Xiaofan,Wang Jinxing,Noriko Yagi.,Yamamoto Yoshimi,Watabe Shoji,Takahashi Y. Sussum,1996，Occurrence of a cathepsin B-like acid cystein proteinase in the eggs of silkworm moth,Antheraea pernyi. Comparative Biochemistry and Physiology. 113B: 95-103.
21. Takahashi S.Y.,Yamamoto Y.,Zhao X-F,Watabe S.,1996,Bombyx acid cysteine proteinase. Invertebrate Reproduction and Development. 30:265-281.
20. Yamamoto Y.,Zhao X-F，Suzuki A.C.,Takahashi. S Y.,1994,Cysteine proteinase from the eggs of the Silkworm,Bombyx mori: site of synthesis and a suggested role in yolk protein degradation. Journal of Insect Physiology. 40: 447-454.
19. Takahashi S.Y.，Zhao X-F，Kageyama T.,Yamamoto Y.,1992，Acid cysteine proteinase from the eggs of silkmoth,Bombyx mori: tissue distribution,developmental changes and the sites of synthesis for the enzyme. Insect Biochemistry and Molecular Biology. 22: 369-377.
18. 赵小凡，2007，昆虫蜕皮的分子机理及应用。昆虫知识，44：323-326。
17. 郑薇薇,张玉宝,王金星,赵小凡*，2007，棉铃虫蜕皮时期同工酶表达模式。昆虫学报，50：317-322。
16．董杜鹃 扈进冬 张新昌 李子劲 王金星 赵小凡* 2006,HCB在Pichia中的表达及鉴定。生物工程杂志，26 : 44-48。
15．赵小凡，基因组时代的昆虫学。2005，3：26-27。
14. 张新昌，刘杨，王金星，赵小凡*,2005，从大豆中纯化棉铃虫组织蛋白酶B抑制因子HCB-SoyI。昆虫学报，48：849-853。
13．杨晓梅，侯立静，董杜鹃，王金星，赵小凡*，2005,组织蛋白酶B在棉铃虫发育过程中的表达及活性研究。山东大学学报. 40：119-124。
12．邵红莲，赵小凡*，董杜鹃，扈进冬，邵丁丁，杨晓梅，王金星，2005，棉铃虫组织蛋白酶B在杆状病毒表达系统中的表达。山东大学学报，40：107-111。
11、杨晓梅，侯立静，康翠洁，邵红莲，张新昌，杜欣军，王金星，赵小凡*,2004，棉铃虫组织蛋白酶B单克隆抗体的制备及鉴定。山东大学学报，39：116-120。
10．安晓萌，杜欣军，邵红莲，邵丁丁，赵小凡*，王金星，2004,棉铃虫组织蛋白酶B多克隆抗体制备及鉴定。昆虫知识，41:132-126。
9．杜欣军，邵丁丁，赵小凡*，王金星，2004,棉铃虫组织蛋白酶B在大肠杆菌中的表达。农业生物技术学报，12：162-166。
8．赵小凡*，蒋晓娟，杜欣军，徐筠娉，王金星，2004，棉铃虫蜕皮调节转录因子在大肠杆菌中的重组表达及包涵体纯化。昆虫学报,47：281-286。
7. 李照民，赵小凡*，王金星，李斐雪，王家宝，王胜利,2003，非甾醇类蜕皮激素竞争物RH-2485对棉铃虫蛋白质组成和表皮结构的影响。动物学研究,24：140-143。
6. 张志宏,赵小凡*,王金星,2002，棉铃虫卵内半胱氨酸蛋白酶的分离纯化、氨基酸序列分析及蛋白酶鉴定。昆虫学报，45：840-843。
5. 张志宏,王金星,赵小凡*,王来元，2002，棉铃虫雌性成虫脂肪体cDNA文库的构建。山东大学学报，37：449-451。
4. 李照民，赵小凡*,李斐雪，王金星，2002，RH-2485对棉铃虫实验室防治效果实验。植物保护学报，29：78-82。
3. 徐夏莲，赵小凡*,王金星，2001，棉铃虫组织蛋白酶B组织分布与合成部位研究。动物学研究，22：242-245。
2. 李斐雪,李照民,张志宏,徐夏莲,赵小凡*,王金星,2001，棉铃虫半胱氨酸蛋白酶底物特异性研究。农业生物技术学报，9：152-155。
1. 赵小凡,2001,昆虫学研究热点述评。《国际学术动态》，2：51-53。
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